Control of thymidine kinase synthesis in IHD vaccinia virus-infected thymidine kinase-deficient LM cells.

PCR detection of thymidine kinase gen of latent herpes simplex Virus type 1 in mice trigeminal ganglia

  Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nuc...

Inhibition of vaccinia virus growth by zinc ions: effects on early RNA and thymidine kinase synthesis.

Accumulation of thymidine kinase activity in vaccinia virus-infected cells was severely inhibited by zinc ions if the drug was added within 1 h postinfection. If added later, zinc ions had no effect on the enzyme synthesis. A fraction of RNA which is normally synthesized in infected cells, was missing from a proper part of the gradient if the cells were treated with zinc ions within 1 h postinf...

Restriction Enzyme Analysis of Thymidine Kinase Gene in Four Iranian Isolates of Herpes Simplex Virus Type 1

Altered properties of thymidine kinase after infection of mouse fibroblast cells with herpes simplex virus.

Previous studies have shown that, after infection by herpes simplex virus (HSV) or vaccinia virus, thymidine kinase activity was induced in either LM mouse fibroblast cells or LM(TK-) cells, a mutant subline lacking this enzyme activity. At 5 to 7 hr after infection, the enzyme activities induced by these viruses in the LM(TK-) cells were about three times as high as those found in exponentiall...

Origin of thymidine kinase in adenovirus-infected human cell lines.

Human adenovirus type 5 enhances the thymidine kinase activity of KB cells but does not induce the enzyme in kinase-deficient HeLa (BU25) cells. Vaccinia induces thymidine kinase activity in both KB and HeLa (BU25) cells. Human adenovirus types 2, 4, 7, and 12 also fail to induce the enzyme in HeLa (BU25) cells. Vaccinia replicates equally well in the presence or absence of HATG (hypoxanthine-a...